Fast Cloning

ClonExpress Ultra One Step Cloning Kit

The ClonExpress cloning technology is simple, fast, and high efficient which enables directional insertion of any amplified DNA product into anylinearized vector at any site. Firstly, the vector is linearized at the cloning site. A small sequence overlapped with each end of the cloning site isadded onto the insert through PCR. The insert and the linearized vector, with overlapped sequences of 15 bp – 20 bp on both 5’- and 3’-end,respectively, are mixed in an appropriate ratio and incubated with recombinase Exnase at 50℃ for 5 – 15 min.The Vazyme ClonExpress UItra One Step Cloning kit is a novel cloning kit, applicable for homologous recombination with 1 – 5 fragments.The Kit is independent of DNA ligase, significantly reducing the self-ligated colonies. The enhanced Exnase and highly optimized bufferincluded in 2X ClonExpress Mix significantly improve the recombination efficiency and the tolerance to impurities. pCE-Zero vector is compatiblewith most PCR products, enabling the specific PCR products to be used directly for recombination without any treatments, which significantlysimplify the procedure.

ClonExpress Entry One Step Cloning Kit

• Easy, fast, and efficient.
• Assemble fragment of length from 50 bp to 10 kb with high efficiency.
• Fragments containing 3’-dA end or bluntend can be directly cloned to CE Entry Vector. No need to add dA.
• Ligase and phosphatase-independent; True positive rate > 95%.
• PCR products can be used directly for cloning without purification.
• Clone can be identified by blue-white screen

Vazyme ClonExpress II One Step Cloning is a simple, fast, and high efficient cloning technology which is based on a homologous recombination technology. It enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly, the vector is linearized at the cloning site. A small sequence overlapped with each end of the cloning site is added onto the insert through PCR. The insert and the linearized vector, with overlapped sequences of 15 bp-20 bp on both 5’- and 3’-end, respectively, are mixed and incubated with Exnase II at 37℃ for 30 min (Fig. 1.). The cloning products can then be directly transformed to competent cells with a ture positive rate > 95%.
ClonExpress Entry One Step Cloning Kit is specially designed for entry cloning based on ClonExpress One Step Cloning technology. The primers of insert should contain 15 bp of constant sequence at 5’-ends homologous to the cloning site of CE Entry Vector provided in this kit. Mix the PCR products of insert and the CE Entry Vector, and incubate for 30 min. Your insert will rapidly and accurately fuse into the vector. During reaction, the homologous recombinase Exnase Entry can catalyze efficiently circularization of amplified insert and CE Entry Vector (up to 100 cfu/ng Vector), with a ture positive rate higher than 95%. In addition, Exnase Entry is compatible with most PCR reaction systems, which means that PCR products can be directly used for recombination without further purification. Compared with TA cloning, the ClonExpress Entry cloning approach is more compatible, more efficient, easier, and is capable of cloning longer fragments.

ClonExpress MultiS One Step Cloning Kit

• Easy, fast, and efficient.
• Directional cloning at any site on any vector.
• No need to consider the restriction enzyme cutting sites carried on inserts.
• Orderly assembly of up to five fragments simultaneously.
• Linearized vector and PCR products can be used directly without purification.

Vazyme ClonExpress One Step Cloning is a simple, fast, and high efficient cloning technology which is based on a homologous recombination technology. It enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly, the vector is linearized at the cloning site. A small sequence overlapped with each end of the cloning site is added onto the insert through PCR. The insert and the linearized vector, with overlapped sequences of 15 bp-20 bp on both 5’- and 3’- end, respectively, are mixed and incubated with Exnase II at 37℃ for 30 min (Fig. 1.). The cloning products can then be directly transformed to competent cells with a positive rate > 95%. 
ClonExpress MultiS One Step Cloning Kit is a new version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS and reaction buffer in this kit are especially optimized for the assembly of multiple fragments. With the help of this kit, up to five fragments can be orderly assembled in a single reaction. In addition, Exnase MultiS is compatible with the reaction systems of restriction enzyme digestion and PCR, which means that both the digestion products of vector and the PCR products of insert can be directly used for recombination without purification, significantly simplifying the procedures.

ClonExpress II One Step Cloning Kit

• Easy, fast, and efficient.
• Directional cloning at any site on any vector.
• No need to consider the restriction enzyme cutting sites carried on inserts.
• Efficient cloning of fragments of 50 bp-10 kb.
• Ligase and phosphatase-independent; True positive rate > 95%.
• Linearized vector and PCR products can be used directly without purification.

Vazyme ClonExpress II One Step Cloning is a simple, fast, and high efficient cloning technology which is based on a homologous recombination technology. It enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly, the vector is linearized at the cloning site. A small sequence overlapped with each end of the cloning site is added onto the insert through PCR. The insert and the linearized vector, with overlapped sequences of 15 bp-20 bp on both 5’- and 3’-end, respectively, are mixed and incubated with Exnase II at 37℃ for 30 min (Fig. 1.). The cloning products can then be directly transformed to competent cells with a true positive rate > 95%.
This kit contains Exnase II and an optimized buffer with a unique recombinant enhancer which significantly improves the recombination efficiency. In addition, Exnase II is compatible with the reaction systems of restriction enzyme digestion and PCR, which means that both the digestion products of vector and the PCR products of insert can be directly used for recombination without purification, significantly simplifying the procedures.