PolyPlus jetMESSENGER® mRNA transfection reagent for hard to transfect cells
- Unmatched transfection efficiency
- High efficiency on a wide variety of difficult to transfect cells
- Outperform DNA transfection by switching to mRNA
- Extremely gentle on cells
- No risk of genome integration
- Perfectly suited for CRISPR/Cas9 gene editing, iPS generation, stem cell differentiation and immunotherapy assays
Specifications
| Reagent | jetMESSENGER® |
| Molecule delivered | mRNA |
| Applications | Gene expression Genome Editing Stem cell differentiation Reprogramming Immunotherapy |
| Cell types | Hard to transfect cells Adherent and suspension cells Primary cells (Neurons, Stem cells, Immune cells, fibroblasts, …) Post-mitotic cells |
| Number of transfections | 1.5 ml of jetMESSENGER® transfection reagent is sufficient to perform up to 1500 transfections in 24-well plates or 375 transfections in 6-well plates |
| Storage | 4 °C, stable for at least one year when stored appropriately |
| Provided with | mRNA buffer |
Summary
Take your gene expression to the next level by switching to mRNA!
Primary cells, neurons, suspension cells and various cancer cell lines are especially challenging to transfect. jetMESSENGER® gives high transfection efficiency in all of these usually difficult to transfect cells, by allowing highly efficient mRNA transfection.
mRNA transfection is as easy as DNA transfection, with the advantage that mRNA does not need to reach the cell nucleus for expression nor require cell division for efficient gene expression. Hence, cells that are slow dividing or that have developed specific mechanisms to protect their genome can finally be used for gene expression.
Unravel the full potential of your cells by switching to mRNA transfection.
Ordering information
| Catalog Number | Amount of reagent | Amount of buffer |
|---|---|---|
| 101000056 | 0.1 ml | 10 ml |
| 101000005 | 0.75 ml | 60 ml |
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