VeriFi® Library Amplification Mix is ideal for NGS library amplification workflows and challenging PCRs. Combining a powerful and robust proofreading enzyme, greatly reduced GC-dependent bias, and AptaLock™ hot start technology, this mix enables precise PCR, regardless the target you are sequencing.
A superior proofreading Pfu polymerase in a specially formulated 2x PCR ready mix designed for NGS library amplification with reduced GC bias. This cutting-edge PCR mix offers market leading performance enabling the acquisition of superior quality datasets with a higher number of unique reads. The mix can be combined with our library quantification kit during NGS library preparation.
Features:
- Low GC bias, ideal for high GC/AT targets
- More unique reads per NGS dataset for superior data quality
- AptaLock™ hot start technology for maximum sensitivity and specificity
- 100x higher fidelity than Taq DNA polymerase
- Room temperature setup
- 2x ready mix for minimal pipetting
Better quality NGS data
VeriFi® Library Amplification Mix was externally validated using Illumina-based sequencing in a blind comparison with three market-leading competitors. VeriFi® Library Amplification Mix consistently gave 2% more uniquely mapped reads than competitors for three different microbial genomes. Depending on sequencing platform being used, this small percentage can translate to 80,000 to 100,000,000 more unique reads. This leads to greater target sequence coverage and more accurate quantitative information, making sequencing experiments more cost-effective and resulting data more informative.
Reduced GC bias during NGS library amplification
Novel buffer composition makes VeriFi® Library Amplification Mix ideal for complex templates. Our research team has challenged this enzyme in PCRs on numerous difficult templates, demonstrating minimal bias during amplification at extremes of GC content ranging from 30% – 70%. With consistently better performance than all major competitors, these results ensure cutting edge performance of the mix regardless of the target sequence, to give you confidence when undertaking costly and laborious NGS experiments. In combination with a higher number of unique reads, discussed above, this feature makes VeriFi® Library Amplification Mix perfect for conducting metagenomic analyses.
PB72.10-01
2x VeriFi™ Library Amplification Mix for NGS, 100×25µl reactions (1 tube of 1.25ml)
PB72.10-05
2x VeriFi™ Library Amplification Mix for NGS, 500×25µl reactions (5 tube of 1.25ml)