CCK-8 Cell Counting Kit (#A311-01)

• Ready-to-use solution.
• High sensitivity, with excellent linear correlation and repeatability.
• Low cytotoxicity

CCK-8 Cell Counting Kit is based on WST-8 [2-(2-methoxy-4-nitrophenyl)-3- (4-nitrophenyl)-5- (2, 4-disulfophenyl)-2H-tetrazolium, monosodium salt] that is rapidly absorbing, highly sensitive and widely used in detection of cell proliferation and cytotoxicity. WST-8, similar to MTT, reduces to a water-soluble formazan dye in the presence of the electron carrying dehydrogenases found in mitochondria. The amount of formazan dye generated by dehydrogenases in living cells is directly proportional to the number of living cells. Measure the Optical Density at 450 nm, using a microplate reader. The measurements can be indirectly shows the number of living cells. CCK-8 Cell counting Kit is a ready-to-use solution, can be directly added to cell supernatants, and incubated for a certain period of time and then test.