T4 DNA ligase
(#1004-200, 1004-1000)
T4 DNA ligase is purified from E.coli stream carrying a plasmid with a cloned gene 30 of phage T4.
Enzyme catalyzes the formation of phosphodiester bonds between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA with blunt or cohesive ends.
Enzyme requires ATP as a cofactor for activity. The presence of NaCl and KCl in concentrations above 200mM inhibits the activity of T4 DNA ligase.
Application
● fast ligation of DNA fragments (cohesive and blunt ends).
● repairing of single-stranded nicks in duplex DNA, RNA or DNA-RNA hybrids. It has no activity against single-stranded nucleic acids.
Liga5™
(#1108-25, 1108-50, 1108-150)
Liga5™ is a 5-minute ligation kit. For sticky-end or blunt-end DNA.
Advantages of the product
- Fast, 5-minute procedure
- Up to 10 times higher yield compared to traditional ligation methods
Description
The Liga5™ kit contains a T4 phage DNA Ligase that catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA with blunt or cohesive end termini. The enzyme repairs single stranded nicks in duplex DNA, RNA or DNA-RNA hybrids but has no activity on single stranded nucleic acids. The T4 phage ligase is purified from an E. coli bacterial strain carrying a plasmid with cloned gene g30 of bacteriophage T4.
The Liga5™ kit improves on average 10 times the ligation efficiency of blunt-ended nucleic acids and up to 3 times the ligation of the cohesive ends, compared to traditional DNA ligation.
The Liga5™ ligation mix has no activity on single-stranded nucleic acids.