UltraScript™ cDNA Synthesis Kit
UltraScript™ Reverse Transcriptase is a robust and thermostable modified MMLV reverse transcriptase engineered to enhance cDNA synthesis speed and yield with accurate transcript representation.
The enzyme allows highly sensitive reverse transcription of viral RNA sequences, making it an ideal component of RT-qPCR-based detection tests. Available as a stand-alone enzyme, or as a ready mix comprising reverse transcriptase, buffer system and, optionally, with an optimised blend of random hexamers with anchored oligo(dT) primers. This reverse transcriptase provides unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations.
Features
- Unbiased representation of 5’ and 3’ mRNA transcript ends
- Reduced RNase H activity
- Thermostable reverse transcriptase 45 °C to 55 °C
- Advanced RNase inhibitor
- High cDNA yields from as little as 4 pg total RNA
- Accurate reverse transcription of GC-rich templates
- Sensitive detection of low copy number transcripts
- Advanced buffer chemistry including Mg and dNTPs
- Both premixed or separate oligo cDNA synthesis kits available
