Reverse Transcriptase

• • Thermostable reverse transcriptase 45°C to 55°C

• • High cDNA yields from as little as 4pg total RNA

• • Sensitive detection of low copy number transcripts
  • Advanced buffer chemistry including Mg and dNTPs

UltraScript Reverse Transcriptase is a robust and thermostable reverse transcriptase engineered to enhance cDNA synthesis speed and yield with accurate transcript representation. The latest developments in reverse transcriptase technology and buffer chemistry allow for efficient, non-biased and sensitive cDNA synthesis.

The enzyme is supplied with a 5x buffer containing Mg, dNTPs, stabilizers and enhancers, optimised to generate high yield, non-biased cDNA from as little as 4pg RNA. As oligos are not included, UltraScript Reverse Transcriptase provides the convenience and flexibility for users to define their own priming strategy depending on the type of analysis needed. UltraScript Reverse Transcriptase gives exceptional performance with gene-specific primers, oligo-dT and random hexamers to produce high quality cDNA ideal for a variety of downstream applications.

High quality cDNA synthesis for downstream qPCR analysis is essential for successful expression studies. Many factors affect cDNA synthesis including the reverse transcripase, buffer systems, enhancers and priming strategy. The PCRBIO cDNA synthesis mix removes the need for user optimisation of these critical factors.