mRNA Production

T7 mScript™ Standard mRNA Production System  (Manual)
Simply the best way to make in vitro mRNA. Combines the benefits of high-yield transcription with those of post-transcriptional capping and tailing all in one kit. See T7-FlashScribe Transcription Kit, ScriptCap m⁷G Capping System, ScriptCap 2′-O-Methyltransferase Kit and A-Plus Poly(A) Polymerase Tailing Kit on this page.

C-MSC11610	10 reactions
C-MSC100625	25 reactions

Post-Transcriptional Capping

ScriptCap™ m⁷G Capping System  (Manual)

For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, GTP and SAM. Cap 0 (N7-methyl-G) caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods. Use in conjunction with ScriptCap 2′-O-Methyltransferase to produce Cap 1 caps, that which is found on most eukaryotic mRNAs.

C-SCCE0625

25 reactions

ScriptCap™ 2′-O-Methyltransferase Kit  (Manual)

For conversion of RNA containing Cap 0 caps to those containing Cap 1 caps, which are found on most eukaryotic mRNAs. Can be used simultaneously with ScriptCap m⁷G Capping System to produce virtually 100% Cap 1-capped RNA in a single reaction.

C-SCMT0625

25 reactions

ScriptCap™ Cap 1 Capping System  (Manual)

For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, ScriptCap 2′-O-Methyltransferase, GTP and SAM. Either Cap 0 or Cap 1 caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods.

C-SCCS1710

10 reactions

Post-Transcriptional Tailing

A-Plus™ Poly(A) Polymerase Tailing Kit  (Manual)

For post-transcriptional poly(A) tail addition to RNAs. Allows tailing of mRNAs
in vitro, to lengths equivalent to or greater than those found in nature. This cannot be accomplished with co-transcriptional tailing methods.

C-PAP5104H

50 reactions