Tornado™ DNAse
(#1009-10T, 1009-50T)
Tornado™ DNAse (RNase-free) is an endonuclease that digests ssDNA, dsDNA and DNA in DNA-RNA complexes. The enzyme contains an additional domain responsible for the effective binding of a substrate DNA. The result is a versatile nuclease that possesses remarkably higher affinity towards dsDNA, even in the nM range of the DNA concentration.
Applications
- removal of contaminating genomic DNA from RNA samples
- removal DNA matrix post in vitro transcription.
- study of DNA-protein interactions by DNase I footprinting
- preventing false positive PCR results in one-step RT-qPCR
DNAse I
(#1009-10, 1009-100)
DNase (RNase-free) is an endonuclease that digests ssDNA, dsDNA and DNA in DNA-RNA complex. The enzyme activity is strictly dependent on Ca²⁺ and is activated by Mg²⁺ and Mn²⁺ ions.
Enzyme is purified from P.pastoris expressing bovine pancreas DNAse I gene. DNAse I may be used to degrade DNA in applications that are sensitive to the presence of RNAses.
Application
● removal of contaminating genomic DNA from RNA samples.
● DNA labeling by nick-translation.
● studies of DNA-protein interactions by DNase I footprinting.
RNAse A
Nuclease for RNA degradation. DNAse free.
(#1006-10, 1006-50)
RNase A is an endoribonuclease that specifically degrades ssRNA at C and U residues.
The enzyme is active depending on the reaction conditions. At low salt concentration (<100 mM NaCl) RNAse cleaves ssRNA, dsRNA and RNA in DNA-RNA complex. At high salt concentration (>300 mM NaCl) RNAse specifically cleaves ssRNA only.
Enzyme is purified from bovine pancreas extracts. RNAse A may be used to degrade RNA in applications that are sensitive to the presence of DNAses.
Application
● removal of RNA from recombinant protein preparation.
● mapping single-base mutation in DNA or RNA.
● plasmid and genomic DNA preparation.
Exonuclease I
(#1020-1, 1020-5)
Recombinant Exonuclease I removal of nucleotides from ssDNA in the 3’-5’ direction.
Application
● removal of ssDNA with a hydroxyl group at the 3-end
● removing primer residues in the mixture after DNA amplification
● when used simultaneously with alkaline phosphatase, removes primers and nucleotides
Also available: Exonuclease V
Viscolase™
Highly efficient nuclease degrading DNA and RNA. Reduces viscosity of the protein preparations.
(#1010-25, 1010-100)
Viscolase™ is a recombinant nuclease with a broad spectrum of nucleolytic activity. It is encoded by the same gene as Benzonase®. Viscolase™ is produced in a unique yeast protein expression system. This versatile endonuclease attacks and degrades all forms of DNA and RNA (both single and double stranded as well as linear and circular forms).
Viscolase™ maintains its extremely high nucleolytic activity over a wide range of operating conditions.
Applications
• Viscosity reduction in cellular lysates and protein extracts
• Sample preparation for 2D gel electrophoresis
• Removal of nucleic acid contaminants from recombinant protein preparations
Advantages
• Viscolase™ is provided in very high purity (over 99%).
• The purification procedure protects the enzyme against impurities such as other proteins including proteases.
• Dilluted enzyme retains the appropriate activity of the enzyme.